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Results Fragment-based drug design and chemistry Utilizing actives identified in a quantitative high-throughput screen against Schistosoma mansoni TGR 20,35 , 92 commercially available low molecular weight (MW) compounds/fragments were tested by X-ray crystallography 33,34,36

Metabolic analysis Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured with a 24-well XF Extracellular Flux Analyzer (Agilent Technologies) and Seahorse Wave Controller Software (Agilent Technologies), according to the manufacturers instructions

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The basic physiology and pathophysiology of melatonin